I would start by checking the router settings to make sure that connecting the laptop via the WLAN does not result in shifting to a wireless mode that is incompatible with the Zune (e.g. proprietary dynamic turbo modes, 802.11g only modes, using up all the DCHP addresses, etc...)
There was a bug in the early Zune 4/8/80 firmwares that prevented wireless sync with another wireless computer over the same WLAN. Do you have the latest Zune software and firmware updates?
Also, I would check your firewall settings, both for any software firewalls you may have on your computer, and any firewall rules you may have configured in your router (not common). Depending on what IP addresses your laptop uses for the wired and wireless connections, this may or may not be an issue.
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