since you said in the past few days, I'm pretty sure you have rebooted/restarted your machine. So besides trying that, I suggest going into the settings of your Zune software and telling it to "Forget the device". This is what the button is actually called, but I forget exactly where. But it is under settings->device. After that, try unplugging and reconnecting the device. If that still doesn't work, maybe try a different USB port.
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